Composite

Part:BBa_K1493702

Designed by: Michiel Herpers   Group: iGEM14_Wageningen_UR   (2014-10-06)
Revision as of 10:26, 20 October 2014 by M Herpers (Talk | contribs)

Toggle switch part with reporter gene gfp

Part of the BananaGuard toggle switch containing a gfp reporter gene. Combined with the BioBrick BBa_K1493703 this part forms a toggle switch. This BioBrick is tested as the whole toggle switch. The part is assembled with BBa_K1493703 in a pSB3K3 backbone.

http://2014.igem.org/File:Characterization_Toggle_Switch.png

‘’Figure 1. The relative fluorescence unit of each toggle switch state. Fluorescence is measured in duplo of cell cultures carrying the pSB3K3 plasmid with the toggle switch construct (BBa_K1493702, BBa_K1493703) grown in M9 medium containing 500 ng/ml aTc (green), 2 mM IPTG (red) and with no inducer added to the medium (blue).’’


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1903


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Categories
Parameters
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