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Part:BBa_K1333201

Designed by: Yanwen Xie   Group: iGEM14_SYSU-China   (2014-09-09)
Revision as of 08:58, 24 September 2014 by MaryZhang (Talk | contribs)

lambda cI-LGF2(D78A)

Fuse lambda cI with LGF2 mutated from Asp to Ala at site 78 (the Glu which locates after three continuous Ala behind lambda repressor is at site 58). As one of the components in Bacterial Two-hybrid System, it causes little interaction with GAL11P compared to the normalized WT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 712
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 767
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Functional Parameters

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This is a graph of B2H system
[edit]
Categories
//chassis/prokaryote/ecoli
//proteindomain/activation
//proteindomain/binding
Parameters
familywith BBa_K1333200, BBa_K1333202, BBa_K1333203
functionhardly interact with Gal11P
proteinfusion protein of lambda cI and LGF2