Part:BBa_K1336005:Experience

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Applications of BBa_K1336005

Figure 1a - BBa_K1336005 ispB xenobiological module is compatible with Reactive Black 5 dye-contaminated waste waters. Graph showing that E.coli transformed with BBa_K1336005 ispB shows comparable growth to the plasmid-free control in LB media with RB5 dye. OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2.

Figure 1b - BBa_K1336005 ispB xenobiological module is compatible with Acid Orange 7 dye-contaminated waste waters. Graph showing that E.coli transformed with BBa_K1336005 ispB shows comparable growth to the plasmid-free control in LB media with AO7 dye. OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2.

As expected, the sole uninduced presence of BBa_K1336005 in transformed cells seems to have no negative effect on E. coli growth, since the growth of the transformed cells is comparable to that of the plasmid-free control.

In addition to this, the presence of azo-dyes AO7 or RB5 in the medium at the indicated concentrations doesn't seem to disturb the growth of BBa_K1336005-transformed cells either. This shows that the non-induced part would be compatible with the viability of E. coli also in az-dye contaminated media.

Figure 2a - Growth of BBa_K1336005 ispB and BBa_K1336006 LEC-ispB xenobiological module in M9 minimal media Graph showing growth of E. coli transformed with BBa_K1336006 and the effect of IPTG induction, using the part only (without lacI) as a control. . OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2.

Figure 2b - Growth of BBa_K1336005 ispB and BBa_K1336006 LEC-ispB xenobiological module in M9+CAS minimal media Graph showing Graph showing growth of E. coli transformed with BBa_K1336006 and the effect of IPTG induction, using the part only (without lacI) as a control. Minimal media with addition of casamino acids was used to comfirm the auxotrophies of the strain we used. . OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2.