Translational_Unit
Part:BBa_K1344004
Designed by: UI Indonesia 2014 Group: iGEM14_UI-Indonesia (2014-10-05)
malS-HlyA (with RBS)
This part is based on BBa_K523001, modified by deleting the stop codons. It's also fusion with HlyA secretional tag for extracelullar expression.
Starch Hydrolyzed Assay Using Iodine Test
Methods
Upon successful cloning of the three genes into our E.coli, we continued to confirm that all three genes are required have hydrolizing amylum activity on LB starch agar. We using iodine test to staining the amylum, and see the clearzone for some time variables.
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Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1233
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 369
Illegal AgeI site found at 1534 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |