Translational_Unit

Part:BBa_K1344004

Designed by: UI Indonesia 2014   Group: iGEM14_UI-Indonesia   (2014-10-05)
Revision as of 03:26, 18 October 2014 by Sahlan (Talk | contribs)

malS-HlyA (with RBS)

This part is based on BBa_K523001, modified by deleting the stop codons. It's also fusion with HlyA secretional tag for extracelullar expression.

Starch Hydrolyzed Assay Using Iodine Test

Methods
Upon successful cloning of the three genes into our E.coli, we continued to confirm that all three genes are required have hydrolizing amylum activity on LB starch agar. We using iodine test to staining the amylum, and see the clearzone for some time variables.

"800px-Starch1.png"

  • <img src="800px-Starch1.png" />
  • <img src="697px-Starch2.png" />
  • <img src="800px-Starch3.png" />
  • <img src="800px-Starch4.png" />
  • <img src="796px-Starch5.png" />
  • <img src="800px-Starch6.png" />
  • <img src="797px-Slide_7.png" />


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1233
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 369
    Illegal AgeI site found at 1534
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None