Plasmid_Backbone
V0120

Part:BBa_J63010:Design

Designed by: Ira Phillips and Caroline Ajo-Franklin   Group: Silver Lab   (2006-10-18)
Revision as of 16:05, 18 October 2006 by Registry (Talk | contribs)

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Protein fusion vector (Silver lab standard)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3246
    Illegal XbaI site found at 3261
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3246
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal NotI site found at 8
    Illegal NotI site found at 3252
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3246
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 3246
    Illegal XbaI site found at 3261
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3246
    Illegal XbaI site found at 3261
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal NgoMIV site found at 2825
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1393
    Illegal SapI site found at 310


Design Notes

transform into TOP10 survival


Source

synthetized DNA

References