Composite
Part:BBa_K1520508
Designed by: yue wu Group: iGEM14_Nanjing-China (2014-09-23)
PgolTS-golS-PgolB-rbs-luxI-Ter
PgolTS-golS-PgolB-rbs-luxI-Ter If there are gold ions, the luxI will be produced, which will result in the producing of AHL.
From left to right, Marker(DL10000), PgolTS-golS-PgolB-rbs-tetR-Ter The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1416
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 548
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//function/biosynthesis/ahl
//function/cellsignalling
//function/cellsignalling
direction | Forward |
ec_num | none |
kegg | none |
protein | luxI-LVA |
swisspro | P12747 |
tag | LVA |
uniprot | P48445 |