Composite
Part:BBa_K1520023
Designed by: yue wu Group: iGEM14_Nanjing-China (2014-09-22)
Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxR-Ter
Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxR-Ter. It is a positive feedback plasmid using luxR as key to feedback. Introducing IPTG will produce rfp and luxR, and luxR will work back to enhance production of rfp.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2380
Illegal NheI site found at 2403 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1208
Illegal BglII site found at 2222 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3358
[edit]
Categories
Parameters
//cds
//cds/transcriptionalregulator/activator
//cds/transcriptionalregulator/repressor
//function/cellsignalling
//function/regulation/transcriptional
//cds/transcriptionalregulator/activator
//cds/transcriptionalregulator/repressor
//function/cellsignalling
//function/regulation/transcriptional
biology | |
control | IPTG, lacI |
direction | Forward |
ec_num | none |
function | Mixed |
kegg | none |
ligands | HSL |
n/a | constitutive promoter family member |
negative_regulators | |
o_h | |
o_l | |
positive_regulators | |
protein | LuxR |
swisspro | P12746 |
tag | None |
uniprot | P48445 |