Part:BBa_K1328002

HXT1-CMVmini-EGFP-tADH1

HXT-1+EGFP+ADH1 consists of HXT-1+CMV promoter, EGFP and terminator ADH1.

HXT-1+CMV promoter controls EGFP expression under the regulation of glucose.

EGFP is used here for testing of HXT1+CMV promoter and will be replaced by insulin gene.

HXT1-CMVmini-EGFP-tADH1

This is a composite part containing a glucose-sensitive promoter (BBa_K1328000) and a EGFP reporter with a tADH1 transcription terminator.

S. cerevisiae cells transformed with this part was cultured in glucose-free medium with glycerol as carbon-source for 8 hrs. They were then cultured for an additional hour with varying glucose levels (0, 5, 15, 25 mmol/L). A increasing level of GFP expression was observed with increasing levels of glucose concentration, in both microscopy and flow cytometry observations.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 818
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 827
  
• 1000
  COMPATIBLE WITH RFC[1000]