Coding

Part:BBa_K1497001

Designed by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen   Group: iGEM14_TU_Darmstadt   (2014-10-07)
Revision as of 12:05, 11 October 2014 by N.Hummel (Talk | contribs)

CHS - Chalcone Synthase from Gerbera

The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in E. coli the source of Malonyl-CoA is limited.

The iGEM Team TU Darmstadt used the CHS and verified the function in their Naringenin Operon (BBa_K1497007).


Figure 1 Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one Naringenin Calcone.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 525
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 534
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 505
    Illegal NgoMIV site found at 1081
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 454


[edit]
Categories
//cds/biosynthesis
//cds/biosynthesis/anthocyanins
Parameters
n/aCHS - Chalcone Synthase from Gerbera