Composite
Part:BBa_K1462270
Designed by: Chuyao Fan Group: iGEM14_SCUT (2014-10-14)
Gal1+cox6+Hbd+ADH1
This part is constructed as a control group to BBa_K1462120 to identify Hbd has been directed to the mitochondria. The GFP is fused at the C-terminus of the enzyme to report where the enzyme located. Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1068
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Categories
Parameters
//function/biosynthesis/butanol
None |