Composite

Part:BBa_K1462260

Designed by: Chuyao Fan   Group: iGEM14_SCUT   (2014-10-10)
Revision as of 19:05, 17 October 2014 by Fcy (Talk | contribs)

Gal1+cox6+Erg10+cyc1

This part is constructed as a control group to BBa_K1462110 to identify Erg10 has been directed to the mitochondria. The GFP is fused at the C-terminus of Erg10 to report where the enzyme located. Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1378
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
Parameters
None