Coding
comDE nati

Part:BBa_K1351015

Designed by: Judith Heckmann   Group: iGEM14_LMU-Munich   (2014-10-02)
Revision as of 15:28, 6 October 2014 by JudithHeckmann (Talk | contribs)

comDE Quorum Sensing two component system - native

Quorum sensing two component system with native RBS and Terminator (histidine kinase comD, response regulator comE) of Streptococcus pneumoniae (R6) able to sense the competence stimulating peptide CSP.



Used oligonucleotides
Prefix: GATCGAATTCGCGGCCGCTTCTAGAGGAAATAAGGGGAAAGAGTAATGG
Sufix: GATCTACTAGTAGCGGCCGCTGCAGATAGTCCGTACGGGATTCG


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/acomDE Quorum Sensing two component system - native