Part:BBa_B0014

Designed by Reshma Shetty Entered: 2003-01-31

TE from coliphage T7 (+/-)

B0014 number 2

Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.

Usage and Biology

<img src="Include File TE.gif" width="413" height="246">

Above is a picture of the wildtype terminator. This terminator has been altered, see design notes. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters

biology	T7TE (+/-)
direction	Forward
forward_efficiency	0.6[CC]
reversed_version	591
reverse_efficiency	-1.06[CC]