Terminator
Part:BBa_B0014
Designed by: Reshma Shetty Group: Antiquity (2003-07-16)
Designed by Reshma Shetty Entered: 2003-01-31
TE from coliphage T7 (+/-)
B0014
Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
Usage and Biology
<img src="Include File TE.gif" width="413" height="246">Above is a picture of the wildtype terminator. This terminator has been altered, see design notes. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
biology | T7TE (+/-) |
direction | Forward |
forward_efficiency | 0.6[CC] |
reversed_version | 591 |
reverse_efficiency | -1.06[CC] |
[edit]
Categories
Parameters
//direction/bidirectional
//terminator/double
//terminator/double
biology | T7TE-LuxIA |
direction | Bidirectional |
forward_efficiency | 0.604[CC]/0.96[JK] |
reversed_version | 2145 |
reverse_efficiency | 0.86[JK] |