Coding

Part:BBa_K1371002:Design

Designed by: Yiran Wu   Group: iGEM14_SCUT-China   (2014-10-03)
Revision as of 14:23, 17 October 2014 by Invly leung (Talk | contribs)

KS-AT2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 252
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 196
    Illegal NgoMIV site found at 1702
    Illegal NgoMIV site found at 2221
    Illegal NgoMIV site found at 2302
    Illegal AgeI site found at 883
    Illegal AgeI site found at 1321
    Illegal AgeI site found at 1644
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Saccharopolyspora erythraea

References

[1]David E. Cane, Programming of Erythromycin Biosynthesis by a Modular Polyketide Synthase, J. Biol. Chem. (2010) 285:27517-27523.

[2]Blaine A. Pfeifer, Suzanne J. Admiraal, Hugo Gramajo, David E. Cane, Chaitan Khosla. Biosythesis of Complex Polyketides in a Metabolically Engineered Strain of ，E.coli [J]. Science, 2001, 291(44): 1790-1792.

[3]Tae, Hongseok,Development of an Analysis Program of Type I Polyketide Synthase Gene Clusters Using Homology Search and Profile Hidden Markov Model, J. Microbiol. Biotechnol. (2009), 19(2), 140–146.

[4]Patrick Caffrey, Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes Chemistry & Biology 8 (2001) 713-723.