Coding
Part:BBa_K1509002:Design
Designed by: Tong Li Group: iGEM14_NEFU_China (2014-09-26)
Revision as of 13:50, 17 October 2014 by LiTong (Talk | contribs) (Created page with "===Design Notes=== It was attained from ''Bacillus sp''. F2 by using PCR with the total genomic DNA of F2 as template. The two synthesized oligonucleotide primers were shown as b...")
Design Notes
It was attained from Bacillus sp. F2 by using PCR with the total genomic DNA of F2 as template. The two synthesized oligonucleotide primers were shown as below: P1: 5’-AGTCTAGATGTAAGCTCTCTTCCGG-3’ P2:5’-CTCTCGAGCAATAAGGACGCAATG-3’ And the PCR runs under the settings as follows: Temperature Number of cycle 96℃ 15min 1 94℃ 40sec
30
50℃ 60sec 72℃ 4min 72℃ 7min 1 Then it was spliced with the DNA sequence for peptide CDS 7 with a synthetic constitutive promoters CP25.
Sourse
It’s origin is one strain named as Bacillus sp. F2, whose flocculent ability can reach 84%.