Composite
Part:BBa_K1431301
Designed by: Rifei Chen, Yushan Zhang Group: iGEM14_SUSTC-Shenzhen (2014-10-10)
TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein
TRE-3G promoter is a kind of eukaryocyte cell promoters that will be switched on after combine with the compound of Tet-On 3G(BBa_K1431101) protein and doxycycline(Dox) mix. That is a very useful way to control whether protein express or not. Of course PolyA is needful in eukaryocyte cell. There are two BbsI sites between promoter and PolyA which can make the coding sequence seamless gather with them. So we can also test how many nucleotides interval between promoter and coding sequence will make the highest expression efficiency.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |