Regulatory

Part:BBa_K1509001:Design

Designed by: Tong Li   Group: iGEM14_NEFU_China   (2014-09-27)
Revision as of 05:59, 27 September 2014 by Registry (Talk | contribs)

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A bi-directional promoter affected by SmtB protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We got smt O-P and smtA, a fragment coding MT-like to sequester metal ions from smt locus by PCR and overlaped two fragments,smtB-smt O-P and pigment gene.Binding with smtB, smt O-P represses the expression of reporter genes.In the presence of heavy metal,the pigment and SmtA can expression normally.


Source

smt O-P is a part of smt locus from Synechococcus PCC7942.

References