Part:BBa_K1381017
CFY-X6 his
Colicin Fy The CFY is a bacteriocin in the colicin family. It is produced naturally by Y.frederiksenii and target close relatives to that bacteria. One among them is the Y.enterocolitica and our target pathogene. The Colicin Fy will target the cellmembrane of it's target.
The killing system We designed the killing system to secret the colicin Fy with the help of an export tag, the USP45 BBa_K1381018. This part will be without promoter, RBS and export tag. It only contains the colicin Fy and a 6x His-Tag for characterization. It is designed so that you can easily couple another promoter or export tag of your choice. This part contains the restriction sites NgoMIV att the start of the sequence to more easily fuse proteins like an export tag to it. Since it got a NgoMIV site att the start of the sequence it is possible to fuse other sequence if they follow the Freiburg Biobrick standard 25 and contains an AgeI site. However our part is not entirely built in the Freiburg standard Biobrick 25 since it does not contain an AgeI site.
Characterization
Figure 4: The gel contains the following from the left to the right
Protein ladder
Eluent 1 for lysate produced by bacteriocin producing bacteria
Eluent 3 for lysate produced by bacteriocin producing bacteria
Eluent 2 for lysate produced by bacteriocin producing bacteria
Final eluent test-tube 4 for lysate produced by bacteriocin producing bacteria
Final eluent test-tube 6 for lysate produced by bacteriocin producing bacteria
Eluent 2 for lysate produced by the negative control
Eluent 3 for lysate produced by the negative control
Final eluent test-tube 4 for lysate produced by the negative control
Final eluent test-tube 6 for lysate produced by the negative control
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
- 1000COMPATIBLE WITH RFC[1000]
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