Coding
Part:BBa_K1402014
Designed by: Chen Xi Group: iGEM14_NJU-QIBEBT (2014-10-04)
BTE-CDS
Acyl carrier protein (ACP) thioesterase catalyzes the hydrolysis of acyl-ACP into fatty acid and ACP. This reaction removes the coupling between fatty acid biosynthesis and phospholipid biosyntheis, which is a key to obtaining free fatty acids. This thioesterase BTE can produce fatty acids with 12~14 carbon chain length. In previous reserches, thioesterase gene BTE can be expressed in E.coli BL21(DE3) and enhance the synthesis of free fatty acids.This part can increase the productivity of free fatty acids. However,this part can not express itself. You should add promoter, RBS and terminator to it or using other parts we offer.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 484
Illegal BglII site found at 624
Illegal BamHI site found at 73 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1033
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Categories
Parameters
None |