Coding
BFR

Part:BBa_K1438000:Design

Designed by: Johann Bauerfeind   Group: iGEM14_Berlin   (2014-09-13)
Revision as of 08:51, 17 October 2014 by JohannB (Talk | contribs) (→‎Design Notes)


Bacterioferritin (BFR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 34
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 142


Design Notes

The featured N-terminal His-tag makes it easy for all future iGEM teams to purify the construct using Ni-NTA affinity chromatography.

For any metal nanoparticles built inside of BFR it would be beneficary to remove any heme groups in order to prevent unloading of BFR by heme-mediated electron transfer. We constructed such a improved biobrick BBa_K1438001

Source

Bacterioferritin from E. coli Nissle 1917 genomic DNA. Amplified by using PCR and cloned into pQE80L

References