Device

Part:BBa_K1373001:Design

Designed by: Wenxuan Chen   Group: iGEM14_SCAU-China   (2014-09-23)
Revision as of 07:50, 17 October 2014 by Lianglujie (Talk | contribs)

nadE with strong promter and strong RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

cloning via omega-PCR

Primers

F-nadE:ctagctactagagaaagaggagaaaATGACATTGCAACAACAAATAATAAAG

R-nadE:GATGATTTCTGGAAAAAGTAAtactagtagcggccgctgcagtc

Source

the promoter is from iGEM distribution kit and the nadE gene is from the genome of E.coli MG1655

References

1.Yong, X.-Y. et al. Enhancement of bioelectricity generation by cofactor manipulation in microbial fuel cell. Biosensorsand Bioelectronics 56, 19-25, doi:http://dx.doi.org/10.1016/j.bios.2013.12.058 (2014).

2.Chen L, Wang F, Wang X, Liu YG. (2013) Robust one-tube Ω-PCR strategy accelerates precise sequence modification of plasmids for functional genomics. Plant Cell Physiology