Part:BBa_K1420005

merT, mercuric transport protein

Transmembrane mercuric binding gene merT (0.3Kb) encodes MerT, which transports Hg(II) species from the periplasm through the membrane. MerT and gene merT are highlighted in purple in Figure 1. The lower part of the Figure 1 shows the arrangement of mer genes in the operon, and merT is located upstream of merP, another gene that encodes a mercury transport protein.

Figure 1. Model of transmembrane mercuric binding protein MerT. The symbol • indicates a cysteine residue. RSH indicates cytosolic thiol redox buffers such as glutathione. Figure 1 shows the interactions of MerT, in purple, with mercury compounds and other gene products of mer operon. (This figure is adapted from "Bacterial mercury resistance from atoms to ecosystems". Reference: T. Barkay et al. FEMS Microbiology Reviews 27 (2003) 355-384.)

merT is found in the Serratia marcescens plasmid pDU1358 on the other side of the bidirectional promoter opposite of merR. It is 351 base pairs long and it codes for a mercuric transport protein.

Protein

MerT is a 116-residue (12.4 kDa) transmembrane protein. The exact structure isn't known, but the protein is predicted to have three transmembrane helices with a cysteine pair accessible from the periplasmic side and another pair on the cytoplasmic side.

Mechanism

The transport mechanism of ionic mercury from the bacterial cell periplasm to cytosplasm is not completely understood. Hg(II) is transferred from the periplasmic cysteine pair to the cytoplasmic pair, where it may go directly to MerA or transfer to cytoplasmic low-molecular mass thiols.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 45
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 30