Coding
ChlM

Part:BBa_K1326001

Designed by: Leah Simmons   Group: iGEM14_Macquarie_Australia   (2014-10-01)
Revision as of 01:10, 17 October 2014 by Leah.Simmons (Talk | contribs)

ChlM

Mg protoporphyrin IX S-adenosyl methionine O-methyl transferase - Magnesium-protoporphyrin O-methyltransferase (ChlM) [PMID: 12828371; 12489983; 4436384]; ChloroP 1.1 predicts cp location

Operon Usage

PSB1C3 Operon 2.png
This gene has been used in an operon with other genes responsible for catalysing the biosynthesis pathway from Mg-protoporphyrin IX to Protochlorophyllide. CTH1, Plastocyanin, and YCF54 are involved in the oxidative cyclase pathway. ChlM methylates Mg-protoporphyrin IX, facilitating the highly-regulated catalysis of Mg-chelatase. CTH1 catalyses the conversion of Mg-protoporphyrin IX monomethyl into divinyl protochlorophyllide, interacting with YCF54 and Plastocyanin.
The insertion of magnesium is the key component of the chlorophyll biosynthesis pathway.
The plasmid is under the control of the lac promoter.

Structure

Crystal Structure of Cyanobacterial ChlM.png

Figure 1: Crystal structure of ChlM from cyanobacteria Synechocystis sp. [http://www.ncbi.nlm.nih.gov/pubmed/25077963]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 230
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 787
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None