Composite
OmpA-BtGal

Part:BBa_K1489004:Design

Designed by: Iowis Zhu   Group: iGEM14_UMaryland   (2014-10-06)
Revision as of 21:20, 6 October 2014 by Registry (Talk | contribs)

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pBAD-RBS-OmpA-Bovine Galectin-1-B0012 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal XhoI site found at 971
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing this part, had to choose between the pBAD and pLac promoters. Decided to use the pBAD promoter due to tighter regulation; we were unsure of whether the protein was toxic and did not want to have too much leaky expression.


Source

OmpA is from E. coli genome Bovine galectin 1 is from Bos taurus genome.

References