Signalling

Part:BBa_K1541017

Designed by: Daniel Gerngross   Group: iGEM14_ETH_Zurich   (2014-10-06)
Revision as of 21:46, 16 October 2014 by Danger (Talk | contribs)

rhlI with optimized RBS

N-butyryl-L-Homoserine lactone or short C4-HSL

This part contains the part C0170 (RhlI), which is an autoinducer synthesis protein that produces N-butyryl-L-HSL (C4-HSL) which binds to RhlR (C0171), obtained from Pseudomonas aeruginosa. Since this part was used in a low-copy vector, RBS engineering was performed to increase expression levels of this enzyme and thus also improve the production of C4-HSL.

Usage and Biology

Quorum Sensing

RhlI is the autoinducer synthetase from Pseudomonas aeruginosa, which can synthesize the molecule N-butyryl-L-HSL (C4-HSL). C4-HSL can bind to the regulatory protein RhlR (C0171). Once C4-HSL is bound to RhlR it can activate the promoters R0071 or I14017.

RBS Engineering

s Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None