Regulatory
NemR (UIR)

Part:BBa_K1398005

Designed by: Exeter iGEM 2014   Group: iGEM14_Exeter   (2014-08-12)
Revision as of 10:22, 12 August 2014 by Registry (Talk | contribs)

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NemR Upstream Intergenic Region

This sequence is found upstream of several NemR regulated genes. It was created for use as a TNT-detection mechanism. It contains the bases from X+ to the start codon, which include a promoter and RBS. This region should allow regulation of gene preoducts through the detection of TNT. If it is not present NemR will bind to a specified sequence of bases and inhibit transcription. If TNT is not present NemR will not bind and transcription will complete.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None