DNA
Part:BBa_K1431413:Design
Designed by: Fan Jiang, Peng Peng Group: iGEM14_SUSTC-Shenzhen (2014-10-14)
target sequence2 for HBV
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
After we use design the gRNA of HBV-2(BBa_K1431403), we need the corresponding binding sequence DNA to test the efficiency of gRNA to lead a double strands break .
But there are two problem:
First,the DSB of binding sequence is hard to observe in the cell;
Second,the whole HBV genome have a potential dangers in the lab.
So we use the alternative solution.We insert the gRNA binding sequence between the promoter and reporter, transform the expression of DSB of HB V to the change of reporter. The whole target-sequence have a gRNA binding sequence in the middle and have more original sequence in two sides to return the natural state of HBV genome.
Source
we will complete this part later