Composite

Part:BBa_K1316012

Designed by: Joan Cortada Garcia   Group: iGEM14_TU_Delft-Leiden   (2014-09-14)
Revision as of 08:38, 14 September 2014 by Registry (Talk | contribs)

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T7 lacO + mtrCAB

Combination of T7 promoter with the lac operator. For the promoter to be active both T7 RNA polymerase and the lac operon inducer (lactose or an analogue such as IPTG) must be present. This double regulatory system reduces the promoter leakage MtrCAB is an electron transport complex of the bacteria Shewanella oneidensis

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 361


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Parameters
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