Reporter
Part:BBa_J58101
Designed by: Valencia iGEM 2006 Group: iGEM06_Valencia (2006-10-26)
Promoter activated synergistically by cI and CRP with the reporter RFP
A promoter activated synergistically by cI and CRP proteins (part ref. BBa_J58100) with a generator of the reporter RFP (part ref. BBa_I13507). This generator uses the following parts: BBa_B0034 (RBS), BBa_E1010 (mRFP1), and BBa_B0015 (Terminator). With this construction, we obtain an AND gate, with RFP as output, and cI and CRP as inputs.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 11
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 11
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 11
Illegal AgeI site found at 687
Illegal AgeI site found at 799 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//classic/reporter/pret
None |