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Part:BBa_K1442117:Design

Designed by: Iva Burova   Group: iGEM14_Warwick   (2014-10-16)
Revision as of 14:04, 16 October 2014 by Registry (Talk | contribs)

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Sldel+8 3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 24
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 24
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 24
    Illegal NgoMIV site found at 48
    Illegal NgoMIV site found at 77
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 40


Design Notes

Gel electrophoresis showing replication by HCV RdRP of a number of potential RNA promoters. The bands show the amount of replicated product and its approximate length, and quantify the tendency to produce strands of wrong size. SLdel+8 (lane 10) does not perform very well in directing replication of a correct sized template and produces a large amount of faulty products.


Source

Brome Mosaic Virus

References