Composite
Part:BBa_K1453000:Design
Designed by: Renhe Luo Group: iGEM14_SJTU-BioX-Shanghai (2014-10-01)
ssDsbA-mRFP-Lgt-TAL USB-His Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 612
Illegal AgeI site found at 724
Illegal AgeI site found at 1537 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1158
Design Notes
When digest with BsmBI, this part can create two sticky-ends, one can match with the first TAL-Protein DiRepeat (Bba_K747000 to Bba_K747015) at 5` of the sequence, the other can match with the sixth TAL-Protein DiRepeat (Bba_K747080 to Bba_K747095) at 3`. With the rest of TAL-Protein DiRepeats (Bba_K747016 to Bba_K747079), user can synthesize a 14 nucleotide Transactivator-like (TAL) protein to suit for their own connector and design their own polymerization. (Golden Gate Cloning) More details about membrane anchor, please find http://2012.igem.org/Team:SJTU-BioX-Shanghai. More details about TAL and Golden Gate Cloning, please find http://2012.igem.org/Team:Freiburg.
Source
BBa_K771000 BBa_E1010