Composite

Part:BBa_K1453000

Designed by: Renhe Luo   Group: iGEM14_SJTU-BioX-Shanghai   (2014-10-01)
Revision as of 15:09, 1 October 2014 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


ssDsbA-mRFP-Lgt-TAL USB-His Tag

This part is designed as a scaffold of connectee.


The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000 The TAL adapter part contains T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI, in order to be used cooperatively with TAL-Protein DiRepeats (Bba_K747000 to Bba_K747095), which is designed by iGEM12_Freiburg.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 612
    Illegal AgeI site found at 724
    Illegal AgeI site found at 1537
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1158


[edit]
Categories
Parameters
None