Device

Part:BBa_K1475006

Designed by: Daniel Weltz Pedersen   Group: iGEM14_SDU-Denmark   (2014-10-05)
Revision as of 12:33, 16 October 2014 by Danie12 (Talk | contribs)

GFP controlled by TetR+LVA and pTet.

This composite part has GFP controlled by a TetR, p(TetR) expression system. The TetR repressor protein binds to the Tet promoter repressing expression. [1] The tet repressor protein is inhibited by [http://openwetware.org/wiki/ATc ATc (anhydrotetracycline)] or the analogue dox (doxycycline) hereby inducing the expression of GFP.[2]

Please note that this part contains the TetR repressor with LVA rapid degradation tag, for a similar part containing TetR repressor without LVA tag please see Part:BBa_K1475005. The TetR without LVA tag can be found here as a basic part: [https://parts.igem.org/Part:BBa_ K1475003 

Part:BBa_ K1475003].


Usage and Biology

This part can be used to test the expression from the TetR p(TetR) system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1485


References

1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677
2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components
3. Cormack, B.P., Valdivia, R.H., and S. Falkow. FACS-optimized mutants of green fluorescent protein (GFP). Gene 173: 33-38 (1996). http://www.sciencedirect.com/science/article/pii/0378111995006850

[edit]
Categories
Parameters
None