Coding

Part:BBa_K1475003

Designed by: Daniel Weltz Pedersen   Group: iGEM14_SDU-Denmark   (2014-10-05)
Revision as of 20:27, 15 October 2014 by Danie12 (Talk | contribs)

tetracyclin repressor from transposon Tn10

The coding region of the frequently used tetracycline repressor (TetR) Part:BBa_C0040 modified by removing the LVA rapid degradation tag, this will in theory serve to increase the protein half-life and in practice increase the concentration of the protein in the cell. This TetR is the tTA subtype which on binding to the p(TetR) promoter Part:BBa_R0040 blocks transcription. [http://openwetware.org/wiki/ATc ATc (anhydrotetracycline)] binds to the Tet repressor inhibiting its function and hereby releasing the repression on the Tet promoter.


Usage and Biology

In addition to ATc (anhydrotetracycline), dox (Doxycycline) can also be used as inducer. [1]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components
2. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677

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