Composite

Part:BBa_K1433002

Designed by: Hanqing Liu   Group: iGEM14_ZJU-China   (2014-09-28)
Revision as of 16:18, 28 September 2014 by Registry (Talk | contribs)

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attL-J23110-attR

Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1. This part is composed of 2 elements. 1. Promoter (reverse): BBa_J23110, a middle-ground Bacterial constitutive promoter. 2. attL and attR sites: Recognition site for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase. This part promotes transcription and translation upstream gene, and when treat with heterologous of Bxb1 gp35 and gp47, downstream gene will express.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 51
    Illegal NheI site found at 74
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 24
    Illegal BsaI.rc site found at 107


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Categories
Parameters
None