Composite

Part:BBa_K1433001

Designed by: Chaofan Zhang   Group: iGEM14_ZJU-China   (2014-09-28)
Revision as of 10:16, 16 October 2014 by Lilina (Talk | contribs)

attB-J23110-attP

Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1.

composition:

    This part is composed of 2 elements.:
  1. Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter.
  2. attB and attP sites: Recognition site for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.

File:Int-xis-bplr-reverse.gif

This part promotes transcription and translation downstream gene, and when treat with Bxb1 gp35, upstream gene will express.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 56
    Illegal NheI site found at 79
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23
    Illegal BsaI.rc site found at 106


[edit]
Categories
Parameters
None