RNA
DPP-IV
Part:BBa_K1442111
Designed by: Dan Goss Group: iGEM14_Warwick (2014-10-08)
DPP-IV (our siRNA target)
DPP-IV
As proof of concept for our project we envisaged downregulating DPP-IV production by interfering with the translational part of its production, through RNA interference.This is a section of the 3’UTR of the protein dipeptidyl peptidase four (DPP-IV). This contains the target sequence for the siRNA (BBa_K1442113).
The schematic above shows the organisation of the DPP-IV target
Usage
This was used to test the silencing ability of the siRNA for DPP-IV (BBa_K1142113) by cloning the target into the forwards GFP for humans (BBa_K1442106). A decrease in fluorescence would be observed with silencing.
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Parameters
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