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Reporter

Part:BBa_K1399013

Designed by: Anna Stikane   Group: iGEM14_Edinburgh   (2014-09-19)
Revision as of 16:54, 19 September 2014 by Registry (Talk | contribs)

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Plac-RFP(DAS)

Lactose/IPTG inducible promoter with RFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged RFP is degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/rfp
//classic/generator/prct
//classic/reporter/pret
//function/reporter/fluorescence
Parameters
emissionRFP
excitation
tagSsrA-DAS degradation tag