Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Reporter

Part:BBa_K1399012

Designed by: Anna Stikane   Group: iGEM14_Edinburgh   (2014-09-19)
Revision as of 16:44, 19 September 2014 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Plac-RFP(LAA)

Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LAA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/rfp
//classic/generator/prct
//classic/reporter/pret
//function/reporter/fluorescence
Parameters
emissionRFP
excitation
tagSsrA-LAA degradation tag