Regulatory
rev promot

Part:BBa_K1463501

Designed by: Beth Greig   Group: iGEM14_Glasgow   (2014-08-12)
Revision as of 22:06, 15 October 2014 by Scolloms (Talk | contribs)

J23100 promoter in reverse

J23100 Constitutive promoter reverse complement

Usage and Biology

We have used this reversed promoter in our PhiC31 integrase-based genetic switch BBa_K1463000 where it transcribed an upstream reversed copy of the RFP gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
    Illegal NheI site found at 24
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None