Coding

Part:BBa_K1475002:Design

Designed by: Daniel Weltz Pedersen   Group: iGEM14_SDU-Denmark   (2014-08-22)
Revision as of 13:39, 22 August 2014 by Registry (Talk | contribs)

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FAT-2 delta 12 desaturase from C. elegans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 117
    Illegal BamHI site found at 253
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 169
    Illegal SapI site found at 582


Design Notes

We used primers to add prefix and suffix to the coding part.


Source

The cDNA sequence comes from wormbase.org and was generously provided by Julius Fredens from University of Southern Denmark.

References