![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K1329001
Designed by: Daniela Geist Group: iGEM14_Marburg (2014-10-01)
Hag-KpnI
Hag-KpnI consists of the Bacillus subtilis flagellin gene which as designed with a KpnI site to integrate different domains, tags or modules with a Gibson assembly after linearizing the vector with KpnI. Using a shuttle vector for gam-positive bacteria it is possible to integrate the modified flagellin construct into the Bacillus genome considering a special heat shock protocoland specific flanks besides the used gene locus. By engineering new modules into the conserved structure of flagellin Bacillus is able to form flagella build up from those modified flagellins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 588
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Categories
Parameters
None |