Reporter
Part:BBa_J04430:Experience
Designed by: Kristen DeCelle Group: iGEM2005 (2005-06-09)
Applications of BBa_J04430
Warwick iGEM 2014 used this part as a positive control for our experiments investigating promoter strengths for a self replicating RNA strand, using the Hepatitis C Virus NS5B gene encoding RNA dependent RNA polymerase, as the replicating enzyme.This was measured using a tecan Magellan plate reader in which fluorescence in the GFP range was measured over a period of 24 hours with both biological and technical replicates for each transformed cell type.
Results
User Reviews
UNIQa99a3bc920979a7c-partinfo-00000001-QINU
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No review score entered. arandall |
1 mM IPTG induced expression using BL21 E. coli cells produces vibrant green cells from both 08 and 09 distributions. |
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Smelissali |
A lovely vibrant green under UV --Smelissali 19:45, 3 July 2006 (EDT) |
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Antiquity |
This review comes from the old result system and indicates that this part worked in some test. |
UNIQa99a3bc920979a7c-partinfo-00000005-QINU