Device
Part:BBa_K1329002
Designed by: Daniela Geist Group: iGEM14_Marburg (2014-10-07)
Insertion of the Metallothionein Cup-1-1 domain into Bacillus subtilis flagellin
The bacterial flagellum is built up of multiple copies of a protein called flagellin. The structure of this protein is mainly conserved between different species, but the variation of a loop directed to the outside of the flagellum can be observed. As we aim to fish metal ions from solution the bacterial flagellum was chosen as a protein scaffold system. We inserted the metallothionein Cup1-1 from Saccharomyces cerevisiae into the variable loop of B. subtilis flagellin. Cup1-1 can bind up to 7 ions of either copper or silver.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 588
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Categories
Parameters
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