Coding
Part:BBa_K1442100
Designed by: Waqar Yousaf Group: iGEM14_Warwick (2014-10-06)
RdRP
This is RdRP, or RNA dependent RNA polymerase. That is, it produces a protein which can bind to a single-strand RNA fragment via an IRES (internal ribosome entry site), read the RNA, and produce from it the same RNA fragment in the opposite sense. This was fundamental to our 'Replicon' project, because it allows the RNA (introduced via viral chassis in the theoretical or transformation in the practical) to self-replicate within a cell.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 492
Illegal BglII site found at 1340 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 537
[edit]
Categories
Parameters
n/a | RdRP |