Device

Part:BBa_K1329000

Designed by: Daniela Geist   Group: iGEM14_Marburg   (2014-10-01)
Revision as of 19:58, 1 October 2014 by Registry (Talk | contribs)

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StrepDARPidin

StrepDARPidin is a fusion protein combining the monomeric streptavidin from Streptomyces avidinii and the DARPin (Designed Ankrin Repeat Protein) EC1 for targeting a tumor surface marker. Streptavidin monomers build up tetrameric constructs and have high and specific affinity to biotin or Strep-Tags. The DARPin EC1 was designed for an specific binding of EpCAM which is a surface marker of tumor cells derived from epithelial tissue. Our idea was to create a fusion protein which can be used for targeting appropiate cells with increased affinity by creating induced proximity using tetrameric structures. Additionally these can be used for linking biotinylated or Strep-tagged constructs at the same time.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 598
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None