Coding

Part:BBa_K1415001

Designed by: HO, TSUNG YU   Group: iGEM14_NCTU_Formosa   (2014-10-02)
Revision as of 13:56, 15 October 2014 by Alex19950425 (Talk | contribs)

PBAN (Mamestra brassicae)

BM.png

PBAN (Pheromone Biosynthesis Activating Neuropeptide) is one kind of peptides that can activate biosynthesis of pheromones of insects we target. Once a PBAN binds with the G-protein coupled receptor on an insect’s pheromone gland, the signal send by the G-protein coupled receptor activates the kinase and phosphatase, and then kinase and phosphatase can activate enzymes that participate in the biosynthesis of insect pheromone, which will be emitted. https://parts.igem.org/Registry:Feature_requests


So,if we ligate the constitutive promoter and ribosome binding site,we can make our E.coli produce our special peptides constantly.


Target insect:Silkworm (Bombyx mori)

Spread: the place where farmers want to feed.

Characteristics : It is entirely dependent on humans for its reproduction and does not occur naturally in the wild.

Damage: None

Control: Not necessary

Bombyx mori.png
The PCR result of our 9 different kinds of PBAN. Because our PBAN DNA sequence length is around 100~150 bp., the PCR result should be 415~515 bp.




















Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None