Device

Part:BBa_K1342001

Designed by: Naoki Saito,Daiki Haraguchi,Yoshiharu Otaki,Ryuhei Minei   Group: iGEM14_Nagahama   (2014-10-03)
Revision as of 16:05, 15 October 2014 by Naoki (Talk | contribs)

zinTp_AspA


Asp Device image.jpg


ZinTp (pYodA) is a promoter which expresses the downstream gene in the presence of cadmium ion. We assembled ZinTp([http://www.example.com BBa_K1342005]), RBS(BBa_B0034), ApeA(BBa_K1342002), Dter(BBa_0015).




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 179
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1071
    Illegal AgeI site found at 600
    Illegal AgeI site found at 1452
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1111


TLC Assay

Analysis of aspartate from strain E.coli K-12/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, not addition cadmium to previous culture of synthesis medium (diluted 1:50 by synthesis medium) 2, (diluted 1:100 by synthesis medium) 3, add cadmium (250μM) 4, (diluted 1:50 by synthesis medium) 5, (diluted 1:100 by synthesis medium) 6, (diluted 1:200 by synthesis medium)



[edit]
Categories
//cds/enzyme
//function/biosynthesis/ahl
//function/motility
Parameters
direction
protein