![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K1329003
Designed by: Daniela Geist Group: iGEM14_Marburg (2014-10-07)
Hag-D2-Strep
In order to build up flagella usable as protein scaffold we wanted to design a system which makes it possible to switch a high variety of functional modules. For that purpose we designed a flagellin construct containing a Strep-Tag inserted into the D2 domain of Salmonella typhimurium on top of the Bacillus Subtilis flagellin as a linker region. By exposure of the Strep-Tag to the outside of the flagellum it would be possible to connect Streptavidin-hybridized fusion proteins of various functionalities to the flagellum as scaffold increasing the epitope density.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 588
[edit]
Categories
Parameters
None |